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 | Acesso ao texto completo restrito à biblioteca da Biblioteca Rui Tendinha. Para informações adicionais entre em contato com biblioteca@incaper.es.gov.br. |
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Registro Completo |
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Biblioteca(s): |
Biblioteca Rui Tendinha. |
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Data corrente: |
05/07/2020 |
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Data da última atualização: |
05/07/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
MAURASTONI, M.; SÁ-ANTUNES, T. F.; OLIVEIRA, S.; SANTOS, A. M. C.; VENTURA, J. A.; FERNANDES, P. M. B. |
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Afiliação: |
Marlonni Maurastoni, UFES; Tathiana F. Sá?Antunes, UFES; Scarlett A. Oliveira, UFES; Alexandre M. C. Santos, UFES; Jose Aires Ventura, Incaper; Patricia M. B. Fernandes, UFES. |
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Título: |
A multiplex RT-PCR method to detect papaya meleira virus complex in adult pre-flowering plants. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Archives of Virology, v. 165, p. 1211-1214, july 2020. |
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Idioma: |
Inglês |
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Conteúdo: |
Papaya sticky disease (PSD), which can destroy orchards, was first attributed to papaya meleira virus (PMeV). However, the discovery of papaya meleira virus 2 (PMeV2) associated with PSD plants impose the need to detect this viral complex. We developed a multiplex RT-PCR (mPCR) technique capable of detecting two viruses in a single assay from pre-flowering plant samples, which is a useful tool for early diagnosis of PSD. We also determined the limit of detection (LOD) using asymmetric plasmid dilutions of both PMeV and PMeV2, which revealed that a higher titer of one virus prevents detection of the other. Thus, this technique is an alternative method for detecting PMeV and PMeV2 in a single reaction. |
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Palavras-Chave: |
Doença das Planta; Multiplex; Papaya meleira virus (PMeV). |
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Thesagro: |
Carica papaya; Doença de planta. |
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Categoria do assunto: |
H Saúde e Patologia |
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Marc: |
LEADER 01439naa a2200241 a 4500
001 1022233
005 2020-07-05
008 2020 bl uuuu u00u1 u #d
100 1 $aMAURASTONI, M.
245 $aA multiplex RT-PCR method to detect papaya meleira virus complex in adult pre-flowering plants.$h[electronic resource]
260 $c2020
520 $aPapaya sticky disease (PSD), which can destroy orchards, was first attributed to papaya meleira virus (PMeV). However, the discovery of papaya meleira virus 2 (PMeV2) associated with PSD plants impose the need to detect this viral complex. We developed a multiplex RT-PCR (mPCR) technique capable of detecting two viruses in a single assay from pre-flowering plant samples, which is a useful tool for early diagnosis of PSD. We also determined the limit of detection (LOD) using asymmetric plasmid dilutions of both PMeV and PMeV2, which revealed that a higher titer of one virus prevents detection of the other. Thus, this technique is an alternative method for detecting PMeV and PMeV2 in a single reaction.
650 $aCarica papaya
650 $aDoença de planta
653 $aDoença das Planta
653 $aMultiplex
653 $aPapaya meleira virus (PMeV)
700 1 $aSÁ-ANTUNES, T. F.
700 1 $aOLIVEIRA, S.
700 1 $aSANTOS, A. M. C.
700 1 $aVENTURA, J. A.
700 1 $aFERNANDES, P. M. B.
773 $tArchives of Virology$gv. 165, p. 1211-1214, july 2020.
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